International Commission for the Taxonomy of Fungi (ICTF)
International Union of Microbiological Societies (IUMS, Mycology Division)

ISTH Library

Global nutrient profiling by phenotype microarrays: a tool complementing genomic and proteomic studies in conidial fungi.

Atanasova, L., Druzhinina, I.S., 2010
J Zhejiang Univ. Sci. B 11(3)

 Conidial fungi or molds and mildews are widely used in modern
biotechnology as producers of antibiotics and other secondary
metabolites, industrially important enzymes, chemicals and food. They
are also important pathogens of animals including humans and
agricultural crops. These various applications and extremely versatile
natural phenotypes have led to the constantly growing list of complete
genomes which are now available. Functional genomics and proteomics
widely exploit the genomic information to study the cell-wide impact of
altered genes on the phenotype of an organism and its function. This
allows for global analysis of the information flow from DNA to RNA to
protein, but it is usually not sufficient for the description of the
global phenotype of an organism. More recently, Phenotype MicroArray
(PM) technology has been introduced as a tool to characterize the
metabolism of a (wild) fungal strain or a mutant. In this article, we
review the background of PM applications for fungi and the methodic
requirements to obtain reliable results. We also report examples of the
versatility of this tool.

Copyright: Irina Druzhinina & Alexey Kopchinskiy 2004 - 2008