International Commission for the Taxonomy of Fungi (ICTF)
International Union of Microbiological Societies (IUMS, Mycology Division)

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Species diversity of Trichoderma in Poland.

Błaszczyk, L., Popiel, D., Chełkowski, J., Koczyk, G., Samuels, G.J., Sobieralski, K., Siwulski, M., 2011
J Appl Genet. 2011 May;52(2):233-43.

 In the present study, we reinvestigate the diversity of Trichoderma
in Poland utilizing a combination of morphological and
molecular/phylogenetic methods. A total of 170 isolates were collected
from six different substrata at 49 sites in Poland. These were divided
among 14 taxa as follows: 110 of 170 Trichoderma isolates were identified to the species level by the analysis of their ITS1, ITS2 rDNA sequences as: T. harzianum (43 isolates), T. aggressivum (35), T. citrinoviride (11), T. hamatum (9), T. virens (6), T. longibrachiatum (4), T. polysporum (1), and T. tomentosum
(1); 60 isolates belonging to the Viride clade were identified based on
a fragment of the translation-elongation factor 1-alpha (tef1) gene as: T. atroviride (20 isolates), T. gamsii (2), T. koningii (17), T. viridescens (13), T. viride (7), and T. koningiopsis (1). Identifications were made using the BLAST interface in TrichOKEY and TrichoBLAST (
The most diverse substrata were soil (nine species per 22 isolates) and
decaying wood (nine species per 75 isolates). The most abundant species
(25%) isolated from all substrata was T. harzianum.

Copyright: Irina Druzhinina & Alexey Kopchinskiy 2004 - 2008