Species diversity of Trichoderma in Poland.
Błaszczyk, L., Popiel, D., Chełkowski, J., Koczyk, G., Samuels, G.J., Sobieralski, K., Siwulski, M., 2011
J Appl Genet. 2011 May;52(2):233-43.
In the present study, we reinvestigate the diversity of Trichoderma
in Poland utilizing a combination of morphological and
molecular/phylogenetic methods. A total of 170 isolates were collected
from six different substrata at 49 sites in Poland. These were divided
among 14 taxa as follows: 110 of 170 Trichoderma isolates were identified to the species level by the analysis of their ITS1, ITS2 rDNA sequences as: T. harzianum (43 isolates), T. aggressivum (35), T. citrinoviride (11), T. hamatum (9), T. virens (6), T. longibrachiatum (4), T. polysporum (1), and T. tomentosum
(1); 60 isolates belonging to the Viride clade were identified based on
a fragment of the translation-elongation factor 1-alpha (tef1) gene as: T. atroviride (20 isolates), T. gamsii (2), T. koningii (17), T. viridescens (13), T. viride (7), and T. koningiopsis (1). Identifications were made using the BLAST interface in TrichOKEY and TrichoBLAST (http://www.isth.info).
The most diverse substrata were soil (nine species per 22 isolates) and
decaying wood (nine species per 75 isolates). The most abundant species
(25%) isolated from all substrata was T. harzianum.