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Kilavan Kannan

University: University of Madras
Institute: Lifesciences
Department: Centre for Advanced Studies in Botanay
Address: Dr. K. P. Kannan M.Sc., M. Phil., Ph.D., Centre for Advanced tudies in Botnay University of Madras Guindy Campus Chennai 600 025 Tamil NAdu India
Telephone: 91 44 26374734
Fax: 91 44 26374734
Email: kp_kannan2001#yahoo.co.in
Scientific interests: Diversity of Endophytic fungi from medicinal plants, Gymnosperms , Pteridophytes , mosses and Psammophytes
Interest in Trichoderma: As i have been interested as i got several species as endophytes
   
Curriculum Vitae:
Curriculum vitae Dr. K.P. Kannan M.Sc., M. Phil., Ph.D., Centre for Advanced studies in Botany University of Madras Guindy Campus, Chennai - 600 025 Tamilnadu, India. E-mail: kp_kannan2001@yahoo.co.in PH: 91 44 – 263 74 734 ____________________________________________ SUMMARY Proficiency in isolation, identification, preservation and maintanence of Endophytic fungi from Medicinal Plants (Angiosperms), Gymnosperms and Pteridophytes. Proficiency in isolation, identification, preservation and maintanence of Phylloplane fungi, Leaf litter fungi and Soil fungi Proficiency in isolation, identification, preservation and maintanence of mycoflora from different kinds of Storage grains. Screening of Mycotoxins, Screening of Extracellular Enzymes from fungi. Proficiency in Electron Microscopic Techniques (Both Transmission Electron Microscopy and Scanning Electron microscopy). Proficiency in Long time Preservation of Microbes. EDUCATION Ph.D., in Botany (Mycology - Fungal Endophytes), Centre for Advanced Studies in Botany, University of Madras, Chennai, India (1998 -2002) (Appendix – I) M.Phil., in Botany (Mycology - Fungal Endophytes), Centre for Advanced Studies in Botany , University of Madras, Chennai, India (1997-1998). (Appendix –II) M.Sc., in Botany, with specialization in Algology, University of Madras, Chennai, India (1995-1997). B.Sc., in Botany, University of Madras, Chennai, India (1992-1995). RESEARCH PUBLICATIONS 1.K. P. Kannan, N. K. Udaya Prakash and Muthumary, J. 2005. A comparative account on the endophytic mycoflora of Gymnosperms from Bangalore and Singapore. Pollution Research, 2005. Vol 24. 1 –6. 2.N.K. Udaya Prakash., G. Kathiravan., K.P. Kannan., T. Saravanan., B. Sampath kumar., S. Bhuvaneswari and J. Muthumary, 2005. Mycoflora of some marketed cereals and pulses. Pollution Research, 2005. Vol 24. 59 –54 3.A.Paulraj, K.P. Kannan, K.Altaff and Muthumary.J.(2001): Diversity of Mesophilic Fungal Population in Freshwater Aquaculture Ponds in Tamilnadu. J.Convergence, Vol.3, No.1-4, pp. 44-51. Communicated 1.K.P. Kannan and Muthumary J. Diversity of endophytic mycobiota in leaves, petioles and stems of medicinal plants (Solanaceae) from Tamil nadu, India. (Botanical Bulletin of Academia Sinica) 2.K.P. Kannan and Muthumary J. Development of conidiomata in Camarosporium palliatum, Kohlmeyer and Kohlmeyer an endophytic Coelomycete (Czech Mycology) RESEARCH EXPERIENCE For more than 8 years WORKING EXPERIENCE Research Fellow: (October 2002 – Till date) Project entitled "Biodiversity of Fungal Population from vegetable oil Refinery with special reference to Sunflower oil", funded by Department of Science and Technology (DST), Government of India, Centre for Advanced Studies in Botany, University of Madras, Chennai - 600 025. Technical Assistant: (Aug’ 99 – Sept’ 2001) “Studies on Biodiversity and ecology of Phyllosticta (Coelomycete) species in Tamilnadu and preparation of regional genetic resources collection of various species.” University Grants Commission, Government of India, Centre for Advanced Studies in Botany, University of Madras, Chennai - 600 025. AFFILIATED TO PROFESSIONAL SOCIETY · Life member - Mycological Society of India (MSI) · Member in Association of Microbiologist of India (AMI) · Member in Electron Microscopical Society of India (EMI) ACTIVITIES · Worked as a Resource person in the workshop on "Microbial Biotechnology and Bioinformatics" held at the Centre for Advanced Studies in Botany, University of Madras, Chennai - 600 025. TRAINING PROGRAMME / WORKSHOP: · Attended a training course for "Collection, Preservation and Maintenance of Fungal Culture Techniques" (including Lyophilization and Liquid nitrogen fixation) at Microbial Type Culture Collection Centre (MTCC), Institute of Microbial Technology, (IMTECH), Sector 39A, Chandigarh.1-4, Dec 1999. India. · Attended the DBT- CAMS sponsored training Workshop on " Molecular Taxonomy of Symbiotic Fungi” held at the School of life Sciences, Jawaharlal Nehru University, New Delhi, India, during 01 to 12 December - 2003, Techniques learnt: Polymerase Chain reaction (PCR), isolation of DNA by CTAB method, isolation of RNA, 2 D gel electrophoresis, Ampified Ribosomal DNA restriction Analysis (ARDRA), Extraction of Plasmid DNA, Proteome mapping, Immunological techniques: - Immunofloresence, ELISA, Western blotting, Axenic cultivation of symbiotic fungi, Interaction between Eukaryotes and Prokayotes - in vitro studies, In Vitro Interaction of Symbiotic Fungi with Conventional Non - targeted AM Plants, In vitro cultivation of plants and interaction with symbiotic fungi, Estimation of Superoxide Dismutase, Determination of Peroxidative damge and Phophatases Isozyme from Symbiotic Fungi. PAPERS PRESENTED: 1. DEVELOPMENT OF CONIDIOMATA IN CAMAROSPORIUM PALLIATUM AN ENDOPHYTIC COELOMYCETE. - K. P. Kannan, J. Muthumary and G. Kathiravan.p.35, presented at the SILVER JUBILEE of the Mycological Society of India (MSI) and Asian Mycological Congress and International Symposium on “Tropical mycology” held at the Centre for Advanced studies Botany, University of Madras, Chennai, India, during 20-22nd January 1999. 2.DEVELOPMENT OF CONIDIOMATA IN SOME PESTALOTIOPSIS Sp. M. Murugan, G. Kathiravan., R. Senthil Kumaran., K. P. Kannan and J.Muthumary.p30, presented at the 26th Annual meeting of Mycological Society of India ( MSI) and National Symposium on “Mycology at 2000 and Beyond” Sardhar Patel University, Gujarat, during 21-23 Dec. 1999. 3.BIODIVERSITY OF VARIOUS SPECIES OF PHYLLOSTICTA FROM SOUTH INDIA", R. Senthilkumaran, G. Kathiravan, K. P. Kannnan and J. Muthumary.p32 presented at the 27th Annual meeting of Mycological Society of India (MSI) and International Symposium on "Frontiers of Fungal Diversity and Diseases in South - East Asia" held at Department of Botany, DDU Gorakhpur University, Gorakpur, U.P.India, from Feb 9th to 11th, 2001. 4.FREQUENCY ANALYSIS OF ENDOPHYTIC FUNGAL POPULATION FROM SOME MEDICINAL PLANTS IN AND AROUND CHENNAI CITY. -K. P. Kannan and J. Muthumary. p 28. Presented at the 28th Annual meeting of Mycological Society of India (MSI) and National conference on "Fungal Diversity and Biotechnology", held at K. V. Pendharker College, Dombivili, Bombay, Maharastra from 2nd to 4th Feb 2002. 5.ENDOPHYTIC MYCOFLORA OF SOME GYMNOSPERMS FROM SINGAPORE. - K. P. Kannan, N. K. Udaya prakash and Muthumary. J. p. 92. Presented at the National Symposium on " Prospecting of Fungal diversity and emerging technologies" and the 29th Annual meeting of Mycological Society of India, held at the Agharkar Research Institute, Pune, Maharastra, India, during February 6 - 7, 2003. 6.INTERNAL MYCOBIOATA OF PTERIDOPHYTES FROM KOLLI HILLS (EASTERN GATS), TAMIL NADU, - K. P. Kannan, N. K. Udaya Prakash and Muthumary J. p. 9. Presented at the National Symposium on " Recent Trends in Biology, Biodiversity and Biotechnology of Fungi” and 30th Annual meeting of the Mycological Society of India (MSI), held at the Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai, 600 025, India, during February, 5- 6, 2004. 7.SOLAR SALTERNS OF EAST COAST OF INDIA - A MYCOLOGICAL SURVEY - V. Kaviyarasan, N. K. Udaya Prakash, K. P. Kannan. B. Sampath Kumar, S. Bhuvaneswari, T. Saravanan and Lakshminarayanan, p24. Presented at the National Symposium on "Recent Trends in Biology, Biodiversity and Biotechnology of Fungi” and 30th Annual meeting of the Mycological Society of India (MSI), held at the Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai, 600 025, India, during February, 5- 6, 2004. 8.A COMPARATIVE OCCOUNT ON THE ENDOPHYTIC MYCOFLORA OF BANGALORE AND SINGAPORE – K. P. Kannan, N. K. Udaya Prakash and Muthumary, J. p 63. Presented at the International Seminar on Ecotechnology for Sustainable Development –ECOTECH 2004, held at the Post Graduate and Research Department of Zoology, The New College, Chennai 600 014, India, during October 4 – 6, 2004. 9.MYCOFLORA OF SOME MARKETED CEREALS AND PULSES - N. K. Udaya Prakash, Kathiravan, G, K. P. Kannan, T. saravanan, B. Sampathkumar, S. Bhuvaneswari and Muthumary J. p64. Presented at the International Seminar on Ecotechnology for Sustainable Development –ECOTECH 2004, held at the Post Graduate and Research Department of Zoology, The New College, Chennai 600 014, India, during October 4 – 6, 2004. 10.A STUDY ON ENDOPHYTIC MYCOBIOTA OF MEDICINALLY IMPORTANT SOLANACEAE MEMBERS – K.P. Kannan, and Muthumary J. p10 –11. Presented at the National Seminar on “ Recent Advances in Mycology” and 31st Annual meeting of the Mycological Society of India (MSI), held at the Department of Biosciences, Mangalore University, Mangalagangotri, 574 199, Karnataka, India, during December 2–3, 2004. 11.OCCURRENCE OF ENDOPHYTIC FUNGI IN THE GYMNOSPERMS OF BANGALORE - K. P. Kannan, N. K. Udaya Prakash and Muthumary J. p 10 – 11. Presented at the National Seminar on “ Recent Advances in Mycology” and 31st Annual meeting of the Mycological Society of India (MSI), held at the Department of Biosciences, Mangalore University, Mangalagangotri, 574 199, Karnataka, India, during December 2–3, 2004. 12.ENDOPHYTIC MYCOBIOTA OF GYMNOSPERMS FROM AUSTIN, TEXAS, USA – K. P. Kannan, J. Muthumary and Udaya Prakash N. K. p. 56. Presented at the Mycological Society of America., Biodiversity of Southern Appalachian fungi. University of North Carolina, USA, 15-17 Jul’ 2004. 13.MYCOLOGICAL STUDIES ON DROPPINGS OF PTEROPUS GIGANTEUS (INDIAN FLYING FOX) FROM CHENNAI, INDIA AND TADARIDA BRASILIENSIS (MEXICAN FREE-TAILED BAT) FROM AUSTIN, USA. - N.K. Udaya Prakash., S. Bhuvaneswari., K.P.Kannan., and J. Muthumary, 2004. Pan American Aerobiologists Association. San Diego, USA, 17-20 Jun’ 2004. 14.ENDOPHYTIC MYCOBIOTA OF SOME GYMNOSPERMS OF TIRUMALA HILLS.- K.P. Kannan, N.K. Udaya Prakash and J. Muthumary, 2005. National Seminar on “Updates on Microbiology”, Sri Padmavathi Mahila University, Tirupati, Andhra Pradesh, Mar’ 29-30, 2005. SYMPOSIA/CONFERENCES PARTICIPATED 1.IMACA and MSI Meetings and International Symposium on “Ecology of Fungi” held at the Goa University, Goa, India, during 20-22nd Jan.1998, 2.Participated the National Symposium on “Recent Developments in Plant pathology”, held at the Centre for Advanced Studies in Botany, University of Madras, during February 26th –27th 1998. 3.Mycological Society of India and Asian Mycological Congress and International symposium on “Tropical mycology” held at the Centre for advanced studies in Botany, University of Madras, and Chennai, India during 20 - 22nd Jan. 1999. 4.Participated the Seminar on "Issues in Biological Research, Ethical and Legal Issues, Bioethics - New Biosciences", organized by the Department of Zoology, University of Madras, during 12th - 13th January 1999. 5.Participated the Seminar on "Trends in molecular Biology and Biotechnology" held at the Department of Zoology, University of Madras, Guindy campus, Chennai – 600 025, sponsored by British Deputy High Commission, South India, Chennai on 20th August 1999. 6.Participated the National Conference on “Electron Microscopy” held at the Defence Materials Stores and Research Development and Establishment, (DMSRDE) Kanpur, 1-3, Dec.1999. 7.Participated the 26th Annual meeting of Mycological Society of India (MSI) and National Symposium on “Mycology at 2000 and Beyond”, Sardhar Patel University, Gujarat, during 21 - 23 Dec. 1999 8. International Conference on "Unani Systems of Medicine", Organized by Niamath Science Academy at the Kalaivanar Arangam, Chennai on the 28th and 29th October 2000 9.Participated the National Symposium on “Microbes in bioremediation for ecofriendly environment in the new millennium.” Organized by the Centre for Advanced Studies in Botany, University of Madras, Chennai, India, during 6th -7th Jan 2000, 10.27th Annual meeting of Mycological Society of India and International Symposium on "Frontiers of Fungal Diversity and Diseases in south - East Asia" held at the Department of Botany, DDU Gorakhpur University, Gorakpur, U.P.India, during Feb 9th to 11th 2001. 11.International seminar on 'Medicinal Plants - Quality Standardization' organized by Voluntary Health Education Research and Developmental Society (VHERDS), held at the IMAGE Auditorium, MRC Nagar, R.A.Puram, Chennai, during June 9th and 10th 2001. 12.Participated the 53rd Annual Meeting of Indian Phytopathological Society (IPS) (Indian Agricultural Research Institute (IARI), New Delhi) and 12th Annual Meeting of Indian Society of Plant Pathologists (INSOP) (Punjab Agricultural University, Ludhiana) and the National Symposium on "Eco-friendly Approaches for Plant disease management", organized by the Center for Advanced Studies in Botany, University of Madras, Chennai-600 002, from 22nd - 24th January 2001. 13.Participated the National Seminar on "Biotechnological and Immunological Strategies for Research", organized by the Department of Biotechnology, University of Madras, Guindy campus, Chennai-600 025, on 15th and 16th March 2001. 14.Participated the 28th Annual meeting of Mycological Society of India (MSI) and National conference on "Fungal Diversity and Biotechnology", held at K. V. Pendharker College, Dombivili, Bombay, Maharastra from 2nd Feb to 4th Feb 2002. 15.Participated the National Symposium on "Recent Advances in Molecular Genetics”, held at the Department of Genetics, Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus on 19th March 2003. 16.Participated the National Symposium on "Prospecting of Fungal diversity and Emerging Technologies" and the 29th Annual meeting of Mycological Society of India (MSI), held at the Agharkar Research Institute, Pune, Maharastra, India, during February 6 - 7, 2003. 17.Participated the National Seminar on “Climate Change in India and its Impact on Plant Diversity and Productivity’’ held at the Department of Botany, University of Delhi, Delhi, 110 007, organized by the Delhi University Botanical Society, during 22nd and 23rd December 2003. 18.International seminar on Ecotechnology for sustainable Development ECOTECH 2004, organized by the Post Graduate and Research Department of Zoology, The New College, Chennai 600 014, India during 4th to 6th October 2004. 19.Participated the National Symposium on "Recent Trends in Biology, Biodiversity and Biotechnology of Fungi" and the 30th Annual meeting of the Mycological Society of India (MSI), held at he Centre for Advanced Studies in Botany, University of Madras, during February 5 - 6, 2004. 20.Participated the National Seminar on “ Recent Advances in Mycology and 31st Annual meeting of the Mycological Society of India (MSI), held at the Department of Biosciences, Mangalore University, Mangalagangotri, 574 199, Karnataka, India, during December 2 – 3, 2004. PERSONAL DETAILS Date of Birth : 08. 05.1975. Place of Birth : Othakadai, Madurai Age and Sex : 29, Male Marital status : Single Passport No : A 7418157 Residential Address : Dr. K.P.KANNAN, No.2 A Railway Car Shed Complex, Annanoor, Avadi, Chennai - 600 109. TAMILNADU, INDIA Residential Phone : (9144) 26374734 E-mail : kp_kannan2001@yahoo.co.in REFEREES: Prof. J. Muthumary @ Kalaivani Centre for advanced studies in Botany University of Madras Guindy Campus, Chennai - 600 025 Tamilnadu, India. E-mail: mm_j@rediffmail.com Prof. N. Anand D.Sc., Director Centre for Advanced studies in Botany University of Madras Guindy Campus, Chennai - 600 025. E-mail: botany@vsnl.com Prof. B. P. R. Vittal Ph. D., Centre For Advanced Studies in Botany University of Madras, Guindy Campus, Chennai-600 25 Tamilnadu, India. Prof. K. Natarajan Ph.D., Centre for Advanced Studies in Botany University of Madras, Guindy Campus, Chennai 600 025 Tamil Nadu, India Dr. N.K. Udaya Prakash Laboratory Manager Omni Environmental, Inc. 2101, Donley Dr. Ste B-100 Austin, TX 78758 Mobile: (512) 203 6827 Email: nkudayaprakash@yahoo.co Appendix – 1 Doctorate of Philosophy (Ph. D) (August 1998 to December 2002) Title of the thesis: "Biodiversity of Endophytic Mycobiota with Special reference to selected medicinal Plants and some gymnosperms" Name of the Supervisor: Prof. J. Muthumary @Kalaivani Centre for advanced studies in Botany University of Madras, Guindy campus Chennai - 600 025, Tamilnadu, India E-mail: mm_j@redifmail.com Abstract In Part – I of the thesis, a total of Twenty different medicinal plants from Tamil Nadu were screened for endophytic fungal population. Altogether 87 species belonging to 25 genera were recorded. Of these, 9 species in 4 genera belonged to Ascomycotina, 78 species in 21 genera to Deuteromycotina, which included 56 species of Coelomycetes and the remaining 22 to Hyphomycetes. A total of 80 sterile morphospecies were also recorded. Highest number of taxa was recorded from Canthium dicoccum and Solanum torvum (21 each) followed by Madhuca indica (18), Phyllanthus amarus and P. maderaspatensis (17 each); Withania somnifera (16); Abrus precatorius and Melia azedarach (15 each); Solanum nigrum, Wrightia tinctoria and Terminalia arjuna (14 each); Vitex negundo, Solanum trilobatum, Glycosmis pentaphylla, Solanum surattense (13 each); Curcuma longa and Santalum album (11 each); Gloriosa superba and Gymnema sylvestre (10 each) whereas, the plant of Erythroxylum monogynum yielded 9 species. In the 2nd, 15 Gymnosperms collected from two different geographical locations, i.e. Bangalore, India and Singapore were screened for their endophytic fungal population. Among them Seven Gymnosperms are collected from Bangalore and they yielded 24 species of endophytic fungi classified under 13 genera. Maximum number of species recorded are from Araucaria bidwilli, A. cookii, A. cunninghamii and Thuja chinensis (11 each) followed by Cupressus sempervirens and Cycas revoluta (10 each) whereas Juniperus chinensis yielded very low number of species. A total of 36 species of fungi belonging to 16 genera were recorded from Gymnosperms collected from Singapore. Of these, 1 species in 1 genus belonged to Ascomycetes, 35 species in 15 genera to Duteromycotina, which included 13 species of Hyphomycetes and remaining 22 to Coelomycetes. Among the 9 gymnosperms studied for the endophytic species diversity from National Botanical Garden, Singapore, the maximum number of species were recorded from Libocedrus macrolepis (13), followed by Araucaria cunninghamii and Pinus kesiya (12 each), P. caribea and Thuja orientalis (11 each) whereas Araucaria columnaris, Juniperus chinensis, Pinus merkusii and Thuja chinensis yielded very low number of species. Nine Xylarious forms and 24 Sterile morphospecies were also recorded. Appendix -1I Master of Philosophy (M.Phil.,) (1997 to 1998) Title of the Project: “Studies on Camarosporiumpalliatum Kohlmeyer and Kohlmeyer an Endophytic Coelomycete with special reference to the developmental morphology of the conidioma”. Name of the Supervisor: Dr. J. Muthumary @Kalaivani University of Madras, Guindy campus Chennai - 600 025, Tamilnadu, India. Abstract The various stages of developmental morphology of the Conidioma in Camarosporium palliatum Kohlmeyer and Kohlmeyer, an endophytic fungus isolated from Suaeda nudiflora Moq were studied. Germination of the conidia on Distilled water and tap water with 1% sucrose solution were carried out. Cultural characteristics of the fungus on Oat Meal Agar, Potato - Carrot Agar, Potato - dextrose Agar and Czapex - Dox- Agar were done. To study the mode of germination as well as the initial stages of the Pycnidial development slide Culture Technique were prepared using the method of Riddell"s (1950). The Agar Blocks with the conidiomata were trimmed into 1mm square blocks. After the preparation of the specimen blocks sections of 1.5 to 2.5 mm were taken using glass knives on Richert - Jung Ultracut E - Ultra microtome and collected on glass slides and stained with toludine blue and observed under the light microscope to study the various stages of conidioma. The conidia is muriform with 3 tranverse and 1-2 longitudinal or oblique septa, Germ tube polar from the apical and basal end of the conidium. Development of the conidiomatal primordium is by symphogenous method. Cavity formation is by "schizogenous" and "lysigenous process". The cells lining the pycnidial cavity function as conidiogenous cels forming conidia. Conidial productionn by budding like process. Presence of first formed "Temporary conidiogenous cells" and later formed "Permanent conidiogenous cells" were observed. Presence of filiform paraphysis - like structure were also observed. Formation of ostiole by dissolution of inner hyaline layer of the papilla. Techniques Learnt: 1. Isolation technique for obtain an endophytic fungus 2. Slide culture Technique 3. Specimen Preparation for Light Microscopy 4. Sectioning using Reichert - Jung Ultracut E – Ultramicrotome

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