International Commission for the Taxonomy of Fungi (ICTF)
International Union of Microbiological Societies (IUMS, Mycology Division)
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Welcome to Trichoderma taxonomy!

ISTH.info - a website of Trichoderma DNA BarCode

Welcome to ISTH.info, the official website of the International Subcommission on Trichoderma and Hypocrea Taxonomy! ISTH is a Subcommission of the International Commission for the Taxonomy of Fungi (ICTF), a Commission of the Mycology Division of the International Union of Microbiological Societies ( IUMS Mycology Division).

This website is designed as an open platform to gather the most recent expert knowledge on Hypocrea/Trichoderma taxonomy and evolution. We also aim to present a collection of easy tools for quick molecular identification of Hypocrea/Trichoderma based on DNA BarCode.

This website has been updated recently (released on February 11th 2008).

11th International workshop on Trichoderma and Gliocladium

“Molecular mechanisms and applications of Trichoderma for biocontrol in agriculture”

October 12-15, 2010, Haifa, Israel

The workshop will bring together researchers working on basic and applied aspects of biocontrol by Trichoderma. Sequencing of the genomes of T. atrovirideand T. virens (formerly Gliocladium virens) has provided unprecedented new tools to understand the molecular mechanisms of the fungal-fungal and fungal-plant interactions that underlie the use of these organisms for biocontrol. At the same time, there is an urgent need for the industry both to guide and to interact with the basic researchers. Read more!

TrichOKEY 2: The Molecular Barcode Program
Number of Hypocrea/Trichoderma species: / Number of ITS1 and 2 BarCodes: Biodiversity profile
1. Name the query set of sequences Why it is important to give name to the sequence query :
2. Paste ITS1 and 2 sequence(s) in FASTA or text format Formats supported by TrichOKEY :
3. Select mode Display modes of TrichOKEY results : Full Short Fasta
biodiversity | ITS1 and 2 master alignment | disclimer | Help

Keynote Lecture on 4th ISTH Meeting, Vienna, Austria 2006 by Walter GamsHighlights of progress and remaining problems in the taxonomy of Trichoderma by Walter GAMS 

Keynote Lecture on 4th ISTH Meeting, Vienna, Austria 2006

Trichoderma and Hypocrea comprise such important fungi, that very intense international efforts are justified to study the phylogenetic structure of the genus and species in depth. What could be achieved with morphology alone in differentiating species of Trichoderma, was shown by Bissett, mainly in 1991 (keys to the species also by Gams & Bissett, 1998). Since 1996 molecular approaches are coming into the scene and are modifying the phylogenetic views drastically...


SIM 56.jpgHypocrea and Trichoderma studies marking the 90th birthday of Joan M. Dingley

Studies in Mycology, No. 56, 2006
PREFACE

We dedicate this work to Joan Marjorie Dingley on the occasion of her 90th birthday.Joan Dingley was born in Auckland, New Zealand,on 14 May 1916. It is particularly fitting that we recognize the scientific contributions of Miss Dingley in this publication dedicated to the Hypocreales. Her works on the Hypocreales of New Zealand (Dingley 1951 a, b; 1952 a, b; 1953 a, b; 1956 a, b; 1957 a, b) established her as a mycologist of international standing while setting a very high standard of excellence for monographic study of the Hypocreales. Especially noteworthy is her early recognition of the necessity to document the entire lifehistory of pleomorphic ascomycetes...
read preface >>


 DNA BARCODE: Trichoderma identification tools

TrichOKEY:  

Here we presented TrichOKEY v. 2, a DNA oligonucliotide BarCode program for the identification of multiple sequences of Hypocrea and Trichoderma based on diagnostic hallmarks detected within ITS1 and 2 (internal transcribed spacers 1 and 2 of the rRNA gene cluster).

The current version contains newly integrated modules to increase the reliability of the result, which are powered by simultaneous identification of multiple sequences, complementary similarity search tool and has an advanced graphic interface.

Read more in Druzhinina et al., 2005

TrichoBLAST:

TrichoBLAST is a publicly available database supported by sequence diagnosis and the similarity search tools, which covers all genetically characterized species of the genus and contains almost complete sets of five of the most frequently used phylogenetic markers: ITS 1 and 2; two introns (tef1_int4(large), tef1_int5-short) and one exon tef1_exon6(large) of the gene encoding translation elongation factor 1-alpha; and a portion of the exon between the 5th and 7th eukaryotic conserved amino acid motives of subunit 2 of the RNA polymerase  gene (rpb2_exon).
Latest bilbiography
Friedl, M. A., Kubicek, C. P. and I. S. Druzhinina, 2008, Carbon source dependence of conidiation and its photostimulation in Hypocrea atroviridis, AEM, 74:1, 245-250 (10 January, 2008)
Friedl, M. A., Schmoll, M., Kubicek, C. P. and I. S. Druzhinina, 2008, Photostimulation of Hypocrea atroviridis growth occurs due to a cross-talk of carbon metabolism, blue light receptors and response to oxidative stress, Mycrobiology UK (10 January, 2008)
Druzhinina, I. S., LaFe, K., Willinger, B., Komoñ-Zelazowska, M., Ammirati, J., Kubicek, C. P and Rogers, J.D., 2008, An unknown species from Hypocreaceae isolated from human lung tissue of a patient with non-fatal pulmonary fibrosis, Clinical Microbiology Newsletters (10 January, 2008)
Komon-Zelzowska, M., Neuhof, T., Dieckmann, R., Döhren, H., Herrera-Estrella, A., Kubicek, C. P. and I. S. Druzhinina, 2007, The formation of atroviridin by Hypocrea atroviridis is conidiation-associated, and positively regulated by blue light and the G-protein GNA3, Eukaryotic Cell (10 January, 2008)
Komoñ-Zelazowska, M., Bissett, J., Zafari, D., Hatvani, L., Manczinger, L., Woo, S., Lorito, M., Kredics, L., Kubicek, C. P. and I. S. Druzhinina, 2007, Genetically closely related but phenotypically divergent Trichoderma species cause world-wide green mould disease in oyster mushroom farms, AEM, 73:22, 7415-7426 (10 January, 2008)
Latest Species
Trichoderma theobromicola Trichoderma, Pachybasium "A" or Hamatum
Hypocrea crystalligena Hypocreamun, Megalocitrina
Trichoderma pleurotum Pachybasium, Harzianum
Hypocrea viridescens/Trichoderma viridescens Trichoderma, Viride
Hypocrea sp. WJ 2608 Pachybasium, Harzianum
Hypocrea sp. WJ 2701 Pachybasium, Harzianum
Trichoderma sp. MA 3642 Longibrahiatum, Longibrachiatum

Copyright: Irina Druzhinina & Alexey Kopchinskiy 2004 - 2008